Journal Article

Differential Expression of Two Flavonoid 3′-Hydroxylase cDNAs Involved in Biosynthesis of Anthocyanin Pigments and 3-Deoxyanthocyanidin Phytoalexins in Sorghum

Chun-Hat Shih, Ivan K. Chu, Wing Kin Yip and Clive Lo

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 10, pages 1412-1419
Published in print October 2006 | ISSN: 0032-0781
Published online October 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcl003
Differential Expression of Two Flavonoid 3′-Hydroxylase cDNAs Involved in Biosynthesis of Anthocyanin Pigments and 3-Deoxyanthocyanidin Phytoalexins in Sorghum

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Three unique sorghum flavonoid 3′-hydroxylase (F3′H) cDNAs (SbF3H1, SbF3H2 and SbF3H3) were discovered through bioinformatics analysis. Their encoded proteins showed >60% identity to the Arabidopsis TT7 (F3′H) protein. Overexpression of SbF3H1 or SbF3H2 restored the ability of tt7 mutants to produce 3′-hydroxylaed flavonoids, establishing their roles as functional F3′H enzymes. In sorghum mesocotyls, SbF3H1 expression was involved in light-specific anthocyanin accumulation while SbF3H2 expression was involved in pathogen-specific 3-deoxyanthocyanidin synthesis. No SbF3H3 expression was detected in all tissues examined. The sorghum mesocotyls represent a good system for investigation of differential regulation of F3′H genes/alleles responding to different external stimuli.

Keywords: Anthocyanins; 3-Deoxyanthocyanidins; Flavonoid 3′-hydroxylases; Sorghum

Journal Article.  2811 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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