Journal Article

Ferredoxin Limits Cyclic Electron Flow around PSI (CEF-PSI) in Higher Plants—Stimulation of CEF-PSI enhances Non-Photochemical Quenching of Chl Fluorescence in Transplastomic Tobacco

Hiroshi Yamamoto, Hideki Kato, Yuki Shinzaki, Sayaka Horiguchi, Toshiharu Shikanai, Toshiharu Hase, Tsuyoshi Endo, Minori Nishioka, Amane Makino, Ken-ichi Tomizawa and Chikahiro Miyake

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 10, pages 1355-1371
Published in print October 2006 | ISSN: 0032-0781
Published online October 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcl005
Ferredoxin Limits Cyclic Electron Flow around PSI (CEF-PSI) in Higher Plants—Stimulation of CEF-PSI enhances Non-Photochemical Quenching of Chl Fluorescence in Transplastomic Tobacco

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We tested the hypothesis that ferredoxin (Fd) limits the activity of cyclic electron flow around PSI (CEF-PSI) in vivo and that the relief of this limitation promotes the non-photochemical quenching (NPQ) of Chl fluorescence. In transplastomic tobacco (Nicotiana tabacum cv Xanthi) expressing Fd from Arabidopsis (Arabidopsis thaliana) in its chloroplasts, the minimum yield (Fo) of Chl fluorescence was higher than in the wild type. Fo was suppressed to the wild-type level upon illumination with far-red light, implying that the transfer of electrons by Fd-quinone oxidoreductase (FQR) from the chloroplast stroma to plastoquinone was enhanced in transplastomic plants. The activity of CEF-PSI became higher in transplastomic than in wild-type plants under conditions limiting photosynthetic linear electron flow. Similarly, the NPQ of Chl fluorescence was enhanced in transplastomic plants. On the other hand, pool sizes of the pigments of the xanthophyll cycle and the amounts of PsbS protein were the same in all plants. All these results supported the hypothesis strongly. We conclude that breeding plants with an NPQ of Chl fluorescence increased by an enhancement of CEF-PSI activity might lead to improved tolerance for abiotic stresses, particularly under conditions of low light use efficiency.

Keywords: Cyclic electron flow; Ferredoxin; Non-photochemical quenching (NPQ); Photosynthesis; Transplastomic tobacco; Water–water cycle

Journal Article.  10498 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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