Journal Article

<i>Chara</i> Myosin and the Energy of Cytoplasmic Streaming

Keiichi Yamamoto, Kiyo Shimada, Khoji Ito, Saeko Hamada, Akio Ishijima, Takayoshi Tsuchiya and Masashi Tazawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 10, pages 1427-1431
Published in print October 2006 | ISSN: 0032-0781
Published online October 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcl006
Chara Myosin and the Energy of Cytoplasmic Streaming

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Recently, it was found that myosin generating very fast cytoplasmic streaming in Chara corallina has very high ATPase activity. To estimate the energy consumed by this myosin, its concentration in the internodal cells of C. corallina was determined by quantitative immunoblot. It was found that the concentration of Chara myosin was considerably high (200 nM) and the amount of ATP consumed by this myosin would exceed that supplied by dark respiration if all myosin molecules were fully activated by the interaction with actin. These results and model calculations suggested that the energy required to generate cytoplasmic streaming is very small and only one-hundredth of the existing myosin is enough to maintain the force for the streaming in the Chara cell.

Keywords: ATPase; Chara corallina; Cytoplasmic streaming; Maintenance energy; Myosin.

Journal Article.  3663 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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