Journal Article

Active NDH-1 Complexes from the Cyanobacterium <i>Synechocystis</i> sp. Strain PCC 6803

Weimin Ma, Young Deng, Teruo Ogawa and Hualing Mi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 10, pages 1432-1436
Published in print October 2006 | ISSN: 0032-0781
Published online October 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcl008
Active NDH-1 Complexes from the Cyanobacterium Synechocystis sp. Strain PCC 6803

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We identified eight bands by staining native gels for NADPH-nitroblue tetrazolium oxidoreductase activity after electrophoresis of n-dodecyl-β-d-maltoside-treated membranes of Synechocystis sp. strain PCC 6803. Among them, bands A, C, D and E were attributed to the activity of NADPH dehydrogenase (NDH-1). Band A is a highly active supercomplex of NDH-1 (about 1,000 kDa) that was absent in the ΔndhD1/D2 mutant and was suppressed under low CO2. Band C was induced under low CO2 or in the ΔndhD1/D2 mutant and was converted to bands D and E. Bands A and C appear to be an NDH-1L dimer and NDH-1M, respectively, with subunits essential for the activity.

Keywords: Active NDH-1 complexes; Activity staining; Synechocystis sp. strain PCC 6803

Journal Article.  2829 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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