Journal Article

Repression of Nitrate Uptake by Replacement of Asp105 by Asparagine in AtNRT3.1 in <i>Arabidopsis thaliana</i> L.

Tahei Kawachi, Yoshihito Sunaga, Munehiro Ebato, Tetsuya Hatanaka and Hisatomi Harada

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 10, pages 1437-1441
Published in print October 2006 | ISSN: 0032-0781
Published online October 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcl010
Repression of Nitrate Uptake by Replacement of Asp105 by Asparagine in AtNRT3.1 in Arabidopsis thaliana L.

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An Arabidopsis mutant (rnc1) with a mutation at the 313th nucleotide from the translational start site of AtNRT3.1 was isolated. The mutation resulted in the replacement of aspartate by asparagine at the 105th amino acid in a region conserved among higher plants. In the rnc1 mutant, both the nitrate concentrations in plants and the nitrate uptake from the medium were <13% compared with those of the wild type, while AtNRT3.1 mRNA was accumulated similarly and both AtNRT1.1 and AtNRT2.1 mRNA were decreased. These results suggest that the replacement of Asp105 in AtNRT3.1 markedly reduces nitrate uptake and accumulation.

Keywords: Arabidopsis thaliana — AtNRT3.1 — Nitrate uptake — Single nucleotide mutation

Journal Article.  2782 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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