Journal Article

Identification of Active Transposon <i>dTok</i>, a Member of the <i>hAT</i> Family, in Rice

Sunok Moon, Ki-Hong Jung, Do-eun Lee, Wen-Zhu Jiang, Hee Jong Koh, Mun-Hue Heu, Dong Sun Lee, Hak Soo Suh and Gynheung An

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 11, pages 1473-1483
Published in print November 2006 | ISSN: 0032-0781
Published online November 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcl012
Identification of Active Transposon dTok, a Member of the hAT Family, in Rice

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Recent completion of the sequencing of the rice genome has revealed that it contains >40% repetitive sequences, most of which are related to inactive transposable elements. During the molecular analysis of the floral organ number1/multiple pistil 2 (fon1/mp2) mutant, we identified an active transposable element dTok0 that was inserted at the kinase domain of FON1, a homolog of CLAVATA1. Insertion of the element into FON1 generated an 8 bp duplication of its target sites, which is one of the major characteristics of the hAT family of transposons. The dTok0 element was actively transposed out of the FON1 gene, leaving 5–8 bp footprints. Reinsertion into a new location was observed at a low frequency. Analysis of the genome sequence showed that the rice cultivar ‘Nipponbare’ contains 25 copies of dTok elements; similar numbers were present in all the Oryza species examined. Because dTok0 does not encode a transposase, enzyme activity should be provided in trans. We identified a putative autonomous transposon, Tok1 that contains an intact open reading frame of the Ac-like transposase.

Keywords: hAT superfamily; floral organ number 1/multiple pistil 2; Oryza; Rice; Transposon

Journal Article.  6532 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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