Journal Article

Genetic Transformation of <i>Populus trichocarpa</i> Genotype Nisqually-1: A Functional Genomic Tool for Woody Plants

Jingyuan Song, Shanfa Lu, Zenn-Zong Chen, Rodrigo Lourenco and Vincent L. Chiang

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 11, pages 1582-1589
Published in print November 2006 | ISSN: 0032-0781
Published online November 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcl018
Genetic Transformation of Populus trichocarpa Genotype Nisqually-1: A Functional Genomic Tool for Woody Plants

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We report here the Agrobacterium-mediated genetic transformation of Nisqually-1, a Populus trichocarpa genotype whose genome was recently sequenced. Several systems were established. Internodal stem segments from vigorously growing greenhouse plants are the explants most amenable to transformation. For the most efficient system, approximately 40% of the stem segments infected with pBI121-containing Agrobacterium tumefaciens C58 produced transgenic calli, as confirmed by β-glucuronidase (GUS) staining. The regeneration efficiency of independent transgenic plants was approximately 13%, as revealed by genomic Southern analysis. Some transgenic plants were produced in as little as 5 months after co-cultivation. This system may help to facilitate studies of gene functions in tree growth and development at a genome level.

Keywords: Agrobacterium tumefaciens; Black cottonwood; Functional genomics; Genetic transformation; Nisqually-1; Populus trichocarpa

Journal Article.  4096 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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