Journal Article

Proteome Analysis of Bell Pepper (<i>Capsicum annuum</i> L.) Chromoplasts

Muhammad Asim Siddique, Jonas Grossmann, Wilhelm Gruissem and Sacha Baginsky

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 12, pages 1663-1673
Published in print December 2006 | ISSN: 0032-0781
Published online December 2006 | e-ISSN: 1471-9053 | DOI:
Proteome Analysis of Bell Pepper (Capsicum annuum L.) Chromoplasts

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


Show Summary Details


We report a comprehensive proteome analysis of chromoplasts from bell pepper (Capsicum annuum L.). The combination of a novel strategy for database-independent detection of proteins from tandem mass spectrometry (MS/MS) data with standard database searches allowed us to identify 151 proteins with a high level of confidence. These include several well-known plastid proteins but also novel proteins that were not previously reported from other plastid proteome studies. The majority of the identified proteins are active in plastid carbohydrate and amino acid metabolism. Among the most abundant individual proteins are capsanthin/capsorubin synthase and fibrillin, which are involved in the synthesis and storage of carotenoids that accumulate to high levels in chromoplasts. The relative abundances of the identified chromoplast proteins differ remarkably compared with their abundances in other plastid types, suggesting a chromoplast-specific metabolic network. Our results provide an overview of the major metabolic pathways active in chromoplasts and extend existing knowledge about prevalent metabolic activities of different plastid types.

Keywords: Biosynthesis; Carotenoid; Chromoplast; Mass spectrometry; Metabolism; Proteomics

Journal Article.  6222 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.