Journal Article

Expression of B2-Type Cyclin-Dependent Kinase is Controlled by Protein Degradation in <i>Arabidopsis thaliana</i>

Sumiko Adachi, Hirofumi Uchimiya and Masaaki Umeda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 47, issue 12, pages 1683-1686
Published in print December 2006 | ISSN: 0032-0781
Published online December 2006 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcl034
Expression of B2-Type Cyclin-Dependent Kinase is Controlled by Protein Degradation in Arabidopsis thaliana

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The eukaryotic cell cycle is controlled by cyclin-dependent kinases (CDKs). Plants possess six types of CDK, among which the B-type CDK (CDKB) is expressed specifically from the late S- to the M-phase. We demonstrate that the expression of Arabidopsis CDKB2 is under the control of the protein degradation machinery. β-Glucuronidase fused to a putative PEST motif of CDKB2 was unstable in tobacco Bright Yellow-2 cells and Arabidopsis plants, and its degradation was arrested by the proteasome inhibitor MG132. We propose that the abundance of CDKB2 protein is regulated not only at the transcriptional level, but also through proteasome-mediated protein degradation.

Keywords: Arabidopsis thaliana; Cyclin-dependent kinase; PEST motif; Protein degradation

Journal Article.  2472 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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