Journal Article

Cellulose Synthesis is Required for Deposition of Reticulate Wall Ingrowths in Transfer Cells

Mark J. Talbot, Geoffrey O. Wasteneys, Christina E. Offler and David W. McCurdy

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 1, pages 147-158
Published in print January 2007 | ISSN: 0032-0781
Published online January 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcl046
Cellulose Synthesis is Required for Deposition of Reticulate Wall Ingrowths in Transfer Cells

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Despite the recognized physiological importance of transfer cells, little is known about how these specialized cells achieve localized deposition of cell wall material, leading to amplification of plasma membrane surface area and enhanced membrane transport capacity. This study establishes that cellulose synthesis is a key early factor in the construction of ‘reticulate’ wall ingrowths, an elaborate but common form of localized wall deposition characteristic of most transfer cells. Using field emission scanning electron microscopy, wall ingrowths were first visible in epidermal transfer cells of Faba bean cotyledons as raised ‘patches’ of disorganized and tangled cellulosic material, and, from these structures, ingrowths emerged via further deposition of wall material. The cellulose biosynthesis inhibitors 2,6-dichlorobenzonitrile and isoxaben both caused dramatic reductions in the number of cells depositing wall ingrowths, altered wall ingrowth morphology and visibly disrupted microfibril structure. The restriction of cellulose deposition to discrete patches suggests a novel mechanism for cellulose synthesis in this circumstance. Overall, these results implicate a central role for cellulose synthesis in reticulate wall ingrowth morphology, especially at the initial stage of ingrowth formation, possibly by providing a template for the self-assembly of wall polymers.

Keywords: Cellulose microfibril; Field emission scanning electron microscopy; Transfer cell; Vicia faba; Wall ingrowth

Journal Article.  6675 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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