Journal Article

Temporary Expression of the <i>TAF10</i> Gene and its Requirement for Normal Development of <i>Arabidopsis thaliana</i>

Yosuke Tamada, Kazuki Nakamori, Hiromi Nakatani, Kentaro Matsuda, Shingo Hata, Tsuyoshi Furumoto and Katsura Izui

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 1, pages 134-146
Published in print January 2007 | ISSN: 0032-0781
Published online January 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcl048
Temporary Expression of the TAF10 Gene and its Requirement for Normal Development of Arabidopsis thaliana

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TAF10 is one of the TATA box-binding protein (TBP)-associated factors (TAFs) which constitute a TFIID with a TBP. Initially most TAFs were thought to be necessary for accurate transcription initiation from a broad group of core promoters. However, it was recently revealed that several TAFs are expressed in limited tissues during animal embryogenesis, and are indispensable for normal development of the tissues. They are called ‘selective’ TAFs. In plants, however, little is known as to these ‘selective’ TAFs and their function. Here we isolated the Arabidopsis thaliana TAF10 gene (atTAF10), which is a single gene closely related to the TAF10 genes of other organisms. atTAF10 was expressed transiently during the development of several organs such as lateral roots, rosette leaves and most floral organs. Such an expression pattern was clearly distinct from that of Arabidopsis Rpb1, which encodes a component of RNA polymerase II, suggesting that atTAF10 functions in not only general transcription but also the selective expression of a subset of genes. In a knockdown mutant of atTAF10, we observed several abnormal phenotypes involved in meristem activity and leaf development, suggesting that atTAF10 is concerned in pleiotropic, but selected morphological events in Arabidopsis. These results clearly demonstrate that TAF10 is a ‘selective’ TAF in plants, providing a new insight into the function of TAFs in plants.

Keywords: Arabidopsis thaliana; General transcription factor; Morphogenesis; TAF10; T-DNA insertion mutant

Journal Article.  6938 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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