Journal Article

Two Novel Nuclear Genes, <i>OsSIG5</i> and <i>OsSIG6</i>, Encoding Potential Plastid Sigma Factors of RNA Polymerase in Rice: Tissue-Specific and Light-Responsive Gene Expression

Yoshiki Kubota, Akio Miyao, Hirohiko Hirochika, Yuzuru Tozawa, Hiroyuki Yasuda, Yuichi Tsunoyama, Yasuo Niwa, Sousuke Imamura, Makoto Shirai and Munehiko Asayama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 1, pages 186-192
Published in print January 2007 | ISSN: 0032-0781
Published online January 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcl050
Two Novel Nuclear Genes, OsSIG5 and OsSIG6, Encoding Potential Plastid Sigma Factors of RNA Polymerase in Rice: Tissue-Specific and Light-Responsive Gene Expression

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Two novel nuclear genes, OsSIG5 and OsSIG6, encoding potential plastid sigma factors of RNA polymerase (RNAP) were identified in Oryza sativa. The deduced amino acid sequences contain conserved regions, regions 1.2–4.2, and a novel region A/B at the N-terminus. Tissue-specific and light-responsive transcripts of OsSIG5 and OsSIG6 were observed. The N-terminal region of OsSig5 conferred import of green fluorescent protein into the chloroplast. Specific transcripts of rice psbA were synthesized in vitro by reconstituted OsSig5–RNAP holoenzymes. These results indicated that OsSig5 is a plastid sigma factor. This is the first report of the Sig5-type sigma factor in crops.

Keywords: Blue/red-light responsive expression; Chloroplast RNA polymerase; Oryza sativa; Promoter; Sigma factor; Transcription

Journal Article.  3918 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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