Journal Article

Bowman–Birk Proteinase Inhibitor Confers Heavy Metal and Multiple Drug Tolerance in Yeast

Nobukazu Shitan, Ken-ichi Horiuchi, Fumihiko Sato and Kazufumi Yazaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 1, pages 193-197
Published in print January 2007 | ISSN: 0032-0781
Published online January 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcl051
Bowman–Birk Proteinase Inhibitor Confers Heavy Metal and Multiple Drug Tolerance in Yeast

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Cultured Coptis japonica cells show tolerance to various toxic compounds. By yeast functional screening of cadmium (Cd) plates with its cDNA library, we isolated a gene encoding Bowman–Birk proteinase inhibitor (CjBBI). The yeast transformant of CjBBI showed multiple tolerance to various drugs adding to Cd, and revealed reduced Cd accumulation in cells. Preferential organs for Cjbbi expression were aerial parts of intact plants, and the subcellular localization of CjBBI was shown, using its green fluorescent protein fusion, to be the apoplast. Induction of Cjbbi expression by Cd treatment suggested that CjBBI was responsible for the tolerance to Cd observed in C. japonica cells.

Keywords: Alkaloid; Cadmium; Coptis japonica; Functional screening; Multiple drug tolerance – Proteinase inhibitor

Journal Article.  2540 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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