Journal Article

Glucose-6-Phosphate Dehydrogenase Plays a Pivotal Role in Nitric Oxide-Involved Defense Against Oxidative Stress Under Salt Stress in Red Kidney Bean Roots

Yinggao Liu, Ruru Wu, Qi Wan, Gengqiang Xie and Yurong Bi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 3, pages 511-522
Published in print March 2007 | ISSN: 0032-0781
Published online March 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm020
Glucose-6-Phosphate Dehydrogenase Plays a Pivotal Role in Nitric Oxide-Involved Defense Against Oxidative Stress Under Salt Stress in Red Kidney Bean Roots

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The pivotal role of glucose-6-phosphate dehydrogenase (G-6-PDH)-mediated nitric oxide (NO) production in the tolerance to oxidative stress induced by 100 mM NaCl in red kidney bean (Phaseolus vulgaris) roots was investigated. The results show that the G-6-PDH activity was enhanced rapidly in the presence of NaCl and reached a maximum at 100 mM. Western blot analysis indicated that the increase of G-6-PDH activity in the red kidney bean roots under 100 mM NaCl was mainly due to the increased content of the G-6-PDH protein. NO production and nitrate reductase (NR) activity were also induced by 100 mM NaCl. The NO production was reduced by NaN3 (an NR inhibitor), but not affected by Nω-nitro-l-arginine (l-NNA) (an NOS inhibitor). Application of 2.5 mM Na3PO4, an inhibitor of G-6-PDH, blocked the increase of G-6-PDH and NR activity, as well as NO production in red kidney bean roots under 100 mM NaCl. The activities of antioxidant enzymes in red kidney bean roots increased in the presence of 100 mM NaCl or sodium nitroprusside (SNP), an NO donor. The increased activities of all antioxidant enzymes tested at 100 mM NaCl were completely inhibited by 2.5 mM Na3PO4. Based on these results, we conclude that G-6-PDH plays a pivotal role in NR-dependent NO production, and in establishing tolerance of red kidney bean roots to salt stress.

Keywords: Glucose-6-phosphate dehydrogenase; Nitrate reductase; Nitric oxide; Red kidney bean; Salt stress

Journal Article.  6569 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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