Journal Article

Epigenetic Inactivation of <i>Chalcone Synthase-A</i> Transgene Transcription in Petunia Leads to a Reversion of the Post-Transcriptional Gene Silencing Phenotype

Akira Kanazawa, Michael O'Dell and Roger P. Hellens

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 4, pages 638-647
Published in print April 2007 | ISSN: 0032-0781
Published online April 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm028
Epigenetic Inactivation of Chalcone Synthase-A Transgene Transcription in Petunia Leads to a Reversion of the Post-Transcriptional Gene Silencing Phenotype

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Petunia plants that exhibit a white-flowering phenotype as a consequence of chalcone synthase transgene-induced silencing occasionally give rise to revertant branches that produce flowers with wild-type pigmentation. Transcription run-on assays confirmed that the production of white flowers is caused by post-transcriptional gene silencing (PTGS), and indicated that transgene transcription is repressed in the revertant plants, providing evidence that induction of PTGS depends on the transcription rate. Transcriptional repression of the transgene was associated with cytosine methylation at CpG, CpNpG and CpNpN sites, and the expression was restored by treatment with either 5-azacytidine or trichostatin A. These results demonstrate that epigenetic changes occurred in the PTGS line, and these changes interfere with the initiation of transgene transcription, leading to a reversion of the PTGS phenotype.

Keywords: Bisulfite sequencing analysis; Cauliflower mosaic virus 35S promoter; Chalcone synthase; Cytosine methylation; Petunia hybrida; Post-transcriptional gene silencing

Journal Article.  6210 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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