Journal Article

Two Alternatively Spliced Transcripts Generated from <i>OsMUS81</i>, a Rice Homolog of Yeast <i>MUS81</i>, Are Up-Regulated by DNA-Damaging Treatments

Naozumi Mimida, Hiroko Kitamoto, Keishi Osakabe, Marina Nakashima, Yuji Ito, Wolf-Dietrich Heyer, Seiichi Toki and Hiroaki Ichikawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 4, pages 648-654
Published in print April 2007 | ISSN: 0032-0781
Published online April 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm029
Two Alternatively Spliced Transcripts Generated from OsMUS81, a Rice Homolog of Yeast MUS81, Are Up-Regulated by DNA-Damaging Treatments

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

OsMUS81, a rice homolog of the yeast MUS81 endonuclease gene, produced two alternative transcripts, OsMUS81α and OsMUS81β. OsMus81α contained a Helix–hairpin–Helix (HhH) motif at the N- and C-termini, and a conserved XPF-like motif in the center, while the OsMus81β isoform lacked the second HhH motif by alternative splicing of a cryptic intron generating a truncated protein. The two transcripts were induced after DNA-damaging treatments such as high intensity light, UV-C and γ-radiation. The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest that OsMus81 functions in maintaining genome integrity through homologous recombination.

Keywords: Alternative splicing; DNA repair; Homologous recombination; MUS81; Rice; XPF endonuclease superfamily

Journal Article.  3758 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.