Journal Article

Nitric Oxide Inhibits Blue Light-Specific Stomatal Opening Via Abscisic Acid Signaling Pathways in <i>Vicia</i> Guard Cells

Xiao Zhang, Atsushi Takemiya, Toshinori Kinoshita and Ken-ichiro Shimazaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 5, pages 715-723
Published in print May 2007 | ISSN: 0032-0781
Published online May 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm039
Nitric Oxide Inhibits Blue Light-Specific Stomatal Opening Via Abscisic Acid Signaling Pathways in Vicia Guard Cells

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Recent evidence suggests that nitric oxide (NO) acts as an intermediate of ABA signal transduction for stomatal closure. However, NO's effect on stomatal opening is poorly understood even though both opening and closing activities determine stomatal aperture. Here we show that NO inhibits stomatal opening specific to blue light, thereby stimulating stomatal closure. NO inhibited blue light-specific stomatal opening but not red light-induced opening. NO inhibited both blue light-induced H+ pumping and H+-ATPase phosphorylation. The NO scavenger 2-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO) restored all these inhibitory effects. ABA and hydrogen peroxide (H2O2) inhibited all of these blue light-specific responses in a manner similar to NO. c-PTIO partially restored the ABA-induced inhibition of all of these opening responses but did not restore inhibition of the responses by H2O2. ABA, H2O2 and NO had slight inhibitory effects on the phosphorylation of phototropins, which are blue light receptors in guard cells. NO inhibited neither fusicoccin-induced H+ pumping in guard cells nor H+ transport by H+-ATPase in the isolated membranes. From these results, we conclude that both NO and H2O2 inhibit blue light-induced activation of H+-ATPase by inhibiting the component(s) between phototropins and H+-ATPase in guard cells and stimulate stomatal closure by ABA.

Keywords: Abscisic acid; Blue light; H+ pumping; Nitric oxide; Stomatal opening

Journal Article.  5424 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.