Journal Article

Antisense Expression of 3-Oxoacyl-ACP Reductase Affects Whole Plant Productivity and Causes Collateral Changes in Activity of Fatty Acid Synthase Components

Paul O'Hara, Antoni R. Slabas and Tony Fawcett

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 5, pages 736-744
Published in print May 2007 | ISSN: 0032-0781
Published online May 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm041
Antisense Expression of 3-Oxoacyl-ACP Reductase Affects Whole Plant Productivity and Causes Collateral Changes in Activity of Fatty Acid Synthase Components

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  • Biochemistry
  • Molecular and Cell Biology
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Brassica napus cv Westar plants were transformed with 3-oxoacyl-ACP reductase (KR) in antisense orientation, driven by either the cauliflower mosaic virus 35S promoter or a seed-specific acyl carrier protein promoter to determine the effects on plant productivity and on the activity of other fatty acid synthase (FAS) components. In plants with altered KR activity, total seed yield was reduced in all cases. In less severely affected plant lines, seeds had a normal appearance and composition but the yield of seeds was reduced by approximately 50%. In more severely affected lines, reductions in both seed fatty acid content and the number of seeds produced per plant were evident, resulting in a 90% reduction in fatty acid synthesized per plant. These phenotypes were independent of the promoter used. In severely affected lines, a large proportion of seeds showed precocious germination, and these had a reduced oleate content and increased levels of polyunsaturated 18-carbon fatty acids, compared with normal seeds of the same line. This reduction in 18:1 fatty acids was mimicked on imbibition of seeds with a normal appearance, indicating a preferential use of oleate moieties in precocious germination events. The reduction in activity of KR was mirrored for a second fatty acid synthase component, enoyl-ACP reductase, indicating a mechanism to maintain the ratio of fatty acid synthase components throughout embryogenesis.

Keywords: Brassica napus; Enoyl-ACP reductase; Fatty acid synthase; Oil yield; 3-Oxoacyl-ACP reductase; Seed

Journal Article.  5770 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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