Journal Article

Regulatory Properties of Potato–<i>Arabidopsis</i> Hybrid ADP-Glucose Pyrophosphorylase

Tiziana Ventriglia, Miguel A. Ballicora, Pedro Crevillén, Jack Preiss and José M. Romero

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 6, pages 875-880
Published in print June 2007 | ISSN: 0032-0781
Published online June 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm047
Regulatory Properties of Potato–Arabidopsis Hybrid ADP-Glucose Pyrophosphorylase

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  • Biochemistry
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In higher plants, ADP-glucose pyrophosphorylase (ADPGlc-PPase) is a heterotetrameric enzyme comprised of two small and two large subunits. Potato–Arabidopsis hybrid ADPGlc-PPases were generated and their regulatory properties analyzed. We show that ADPGlc-PPase subunits from two different species can interact, producing active enzymes with new regulatory properties. Depending on the subunit combinations, hybrid heterotetramers showed responses to allosteric effectors [3-phosphoglycerate (3-PGA) and Pi] in the micromolar or millimolar range. While hybrid potato small subunit (PSS) and the Arabidopsis large subunit APL1 showed an extremely sensitive response to 3-PGA and Pi, hybrid PSS/Arabidopsis APL2 was very insensitive to them. Intermediate responses were determined for other subunit combinations.

Keywords: ADP-glucose pyrophosphorylase; Allosteric regulation; Arabidopsis thaliana; Heterotetrameric enzyme; Hybrid enzyme; Potato

Journal Article.  4236 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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