Journal Article

Stable Oil Bodies Sheltered by a Unique Oleosin in Lily Pollen

Pei-Luen Jiang, Co-Shing Wang, Chia-Mei Hsu, Guang-Yuh Jauh and Jason T. C. Tzen

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 6, pages 812-821
Published in print June 2007 | ISSN: 0032-0781
Published online June 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm051
Stable Oil Bodies Sheltered by a Unique Oleosin in Lily Pollen

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  • Biochemistry
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Stable oil bodies were purified from mature lily (Lilium longiflorum Thunb.) pollen. The integrity of pollen oil bodies was maintained via electronegative repulsion and steric hindrance possibly provided by their surface proteins. Immunodetection revealed that a major protein of 18 kDa was exclusively present in pollen oil bodies and massively accumulated in late stages of pollen maturation. According to mass spectrometric analyses, this oil body protein possessed a tryptic fragment of 13 residues matching that of a theoretical rice oleosin. A complete cDNA fragment encoding this putative oleosin was obtained by PCR cloning with primers derived from its known 13-residue sequence. Sequence analysis as well as immunological non-cross-reactivity suggests that this pollen oleosin represents a distinct class in comparison with oleosins found in seed oil bodies and tapetum. In pollen cells observed by electron microscopy, oil bodies were presumably surrounded by tubular membrane structures, and encapsulated in the vacuoles after germination. It seems that pollen oil bodies are mobilized via a different route from that of glyoxysomal mobilization of seed oil bodies after germination.

Keywords: Germination; Lily (Lilium longiflorum Thunb.); Oil bodies; Oleosin; Pollen

Journal Article.  4922 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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