Journal Article

Context Analysis of Termination Codons in mRNA that are Recognized by Plant NMD

Koichi Hori and Yuichiro Watanabe

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 7, pages 1072-1078
Published in print July 2007 | ISSN: 0032-0781
Published online July 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm075
Context Analysis of Termination Codons in mRNA that are Recognized by Plant NMD

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

The nonsense-mediated mRNA decay (NMD) system is an RNA surveillance system that degrades mRNAs possessing premature translation termination codons (PTCs). Although NMD factors are well conserved in eukaryotes, it is speculated that the contexts of those termination codons that are subject to NMD are different depending on the organism. Context analysis of termination codons that are recognized by the plant NMD system would clarify NMD target mRNAs in plants, and contribute to our understanding of its biological relevance in plants. In the present study we analyzed the positions of termination codons that were recognized as PTCs using an Agrobacterium transient expression assay, i.e. the accumulation of a series of plant mRNAs with nonsense mutations in different contexts was tested in plants. The results indicated that termination codons that are located distant from the mRNA 3′ termini or >50 nucleotides upstream of the 3′-most exon–exon junction are recognized as substrates for NMD.

Keywords: Premature termination codon; mRNA stability; mRNA surveillance; Nonsense-mediated mRNA decay; Post-transcriptional regulation; Untranslated region

Journal Article.  3586 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.