Journal Article

The Jasmonate-Induced Expression of the <i>Nicotiana tabacum</i> Leaf Lectin

Nausicaä Lannoo, Gianni Vandenborre, Otto Miersch, Guy Smagghe, Claus Wasternack, Willy J. Peumans and Els J. M. Van Damme

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 8, pages 1207-1218
Published in print August 2007 | ISSN: 0032-0781
Published online August 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm090
The Jasmonate-Induced Expression of the Nicotiana tabacum Leaf Lectin

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Previous experiments with tobacco (Nicotiana tabacum L. cv Samsun NN) plants revealed that jasmonic acid methyl ester (JAME) induces the expression of a cytoplasmic/nuclear lectin in leaf cells and provided the first evidence that jasmonates affect the expression of carbohydrate-binding proteins in plant cells. To corroborate the induced accumulation of relatively large amounts of a cytoplasmic/nuclear lectin, a detailed study was performed on the induction of the lectin in both intact tobacco plants and excised leaves. Experiments with different stress factors demonstrated that the lectin is exclusively induced by exogeneously applied jasmonic acid and JAME, and to a lesser extent by insect herbivory. The lectin concentration depends on leaf age and the position of the tissue in the leaf. JAME acts systemically in intact plants but very locally in excised leaves. Kinetic analyses indicated that the lectin is synthesized within 12 h exposure time to JAME, reaching a maximum after 60 h. After removal of JAME, the lectin progressively disappears from the leaf tissue. The JAME-induced accumulation of an abundant nuclear/cytoplasmic lectin is discussed in view of the possible role of this lectin in the plant.

Keywords: Inducible protein; Jasmonate; Lectin; Nicotiana tabacum; Spodoptera littoralis; Tobacco

Journal Article.  8196 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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