Journal Article

Blue Light-Dependent Nuclear Positioning in <i>Arabidopsis thaliana</i> Leaf Cells

Kosei Iwabuchi, Tatsuya Sakai and Shingo Takagi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 9, pages 1291-1298
Published in print September 2007 | ISSN: 0032-0781
Published online September 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm095
Blue Light-Dependent Nuclear Positioning in Arabidopsis thaliana Leaf Cells

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The plant nucleus changes its intracellular position not only upon cell division and cell growth but also in response to environmental stimuli such as light. We found that the nucleus takes different intracellular positions depending on blue light in Arabidopsis thaliana leaf cells. Under dark conditions, nuclei in mesophyll cells were positioned at the center of the bottom of cells (dark position). Under blue light at 100 μmol m−2 s−1, in contrast, nuclei were located along the anticlinal walls (light position). The nuclear positioning from the dark position to the light position was fully induced within a few hours of blue light illumination, and it was a reversible response. The response was also observed in epidermal cells, which have no chloroplasts, suggesting that the nucleus has the potential actively to change its position without chloroplasts. Light-dependent nuclear positioning was induced specifically by blue light at >50 μmol m−2 s−1. Furthermore, the response to blue light was induced in phot1 but not in phot2 and phot1phot2 mutants. Unexpectedly, we also found that nuclei as well as chloroplasts in phot2 and phot1phot2 mutants took unusual intracellular positions under both dark and light conditions. The lack of the response and the unusual positioning of nuclei and chloroplasts in the phot2 mutant were recovered by externally introducing the PHOT2 gene into the mutant. These results indicate that phot2 mediates the blue light-dependent nuclear positioning and the proper positioning of nuclei and chloroplasts. This is the first characterization of light-dependent nuclear positioning in spermatophytes.

Keywords: Arabidopsis thaliana; Blue light; Epidermal cell; Mesophyll cell; Nuclear positioning; Phototropin

Journal Article.  4678 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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