Journal Article

Cyanobacteriochrome TePixJ of <i>Thermosynechococcus elongatus</i> Harbors Phycoviolobilin as a Chromophore

Takami Ishizuka, Rei Narikawa, Takayuki Kohchi, Mitsunori Katayama and Masahiko Ikeuchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 9, pages 1385-1390
Published in print September 2007 | ISSN: 0032-0781
Published online September 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm106
Cyanobacteriochrome TePixJ of Thermosynechococcus elongatus Harbors Phycoviolobilin as a Chromophore

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  • Biochemistry
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Cyanobacteria have several putative photoreceptors (designated cyanobacteriochromes) that are related to but distinct from the established phytochromes. The GAF domain of the phototaxis regulator, PixJ, from a thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 (TePixJ_GAF) is a cyanobacteriochrome which exhibits reversible photoconversion between a blue light-absorbing form (max = 433 nm) and a green light-absorbing form (max = 531 nm). To study the chromophore, we prepared TePixJ_GAF chromoprotein from heterologously expressed Synechocystis and performed spectral analysis after denaturation by comparing it with the cyanobacterial phytochrome Cph1 which harbors phycocyanobilin (PCB) as a chromophore. The results indicated that the chromophore of TePixJ is not PCB, but its isomer, phycoviolobilin (PVB). It is suggested that the GAF domain of TePixJ has auto-lyase and auto-isomerase activities.

Keywords: Auto-isomerase — Cyanobacteria — Cyanobacteriochrome — Phycocyanobilin — Phycoviolobilin — Phytochrome

Journal Article.  2863 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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