Journal Article

Floral Development of an Asexual and Female-Like Mutant Carrying Two Deletions in Gynoecium-Suppressing and Stamen-Promoting Functional Regions on the Y Chromosome of the Dioecious Plant <i>Silene latifolia</i>

Ayako Koizumi, Yasuhito Amanai, Kotaro Ishii, Kiyoshi Nishihara, Yusuke Kazama, Wakana Uchida and Shigeyuki Kawano

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 10, pages 1450-1461
Published in print October 2007 | ISSN: 0032-0781
Published online October 2007 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcm113
Floral Development of an Asexual and Female-Like Mutant Carrying Two Deletions in Gynoecium-Suppressing and Stamen-Promoting Functional Regions on the Y Chromosome of the Dioecious Plant Silene latifolia

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Sexual dimorphism is controlled by genes on the Y chromosome in the dioecious plant Silene latifolia. K034 is the first mutant with female flowers and asexual flowers in one individual. Its stamens are suppressed completely, and its gynoecium exhibits two suppression patterns. One gynoecium resembles a thin rod, as in wild-type males (asexual flower); the other is imperfectly suppressed, having 1–3 carpels (female-like flower). The ratio of these patterns was 9 : 1. To exclude the possibility of chimerism in K034, we crossed a female-like flower of K034 with a wild-type male. Progeny obtained from this crossing had asexual and female-like flowers in one individual. This two-flower-type phenotype was inherited without separating. To examine the identity of flower organs in K034, we analyzed the development of asexual and female-like flowers using scanning electron microscopy and in situ hybridization with SLM1 and SLM2 (orthologs of AGAMOUS and PISTILLATA, respectively) as probes. Mitotic spreads of root tip chromosomes from hairy root cultures showed that K034 had 25 chromosomes. Fluorescent in situ hybridization analysis, using a subtelomeric repetitive sequence (KpnI subfamily) as a probe, indicated that K034 possessed two X chromosomes and one Y chromosome (Yd), of which Yd had been rearranged to lose the pseudoautosomal region (PAR). PCR analysis using Y-specific sequence-tagged site (STS) markers clarified that Yd of K034 had two other deletions in gynoecium-suppressing and stamen-promoting regions. It is reasonable to suggest that these sex chromosomal abnormalities resulted in two abnormal sexual phenotypes: the asexual and imperfect female (female-like) flowers in K034.

Keywords: Silene latifolia; dioecious plant; floral development; asexual flower; female-like flower; Y chromosome

Journal Article.  6602 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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