Journal Article

Isolation of SIP, a Protein that Interacts with SPB, a Possible Transcriptional Regulatory Factor in <i>Rhodobacter sphaeroides</i>

Hiroshi Shimada, Kazuhiro Ishida, Yasuhiro Machiya and Ken-ichiro Takamiya

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 10, pages 1504-1508
Published in print October 2007 | ISSN: 0032-0781
Published online October 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm114
Isolation of SIP, a Protein that Interacts with SPB, a Possible Transcriptional Regulatory Factor in Rhodobacter sphaeroides

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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SPB is a transcriptional factor in Rhodobacter sphaeroides that represses expression of the puf operon under aerobic or semi-aerobic light conditions. Here, we identified a 17,500 Da protein designated SIP (SPB interaction protein) that interacts with SPB, as determined by binding to an SPB-His×6 fusion protein-Ni column. The SPB–SIP interaction in vivo was confirmed by an immunoprecipitation assay. The level of transcripts and protein of SIP did not differ for all growth conditions tested, indicating that regulation of the SIP–SPB interaction, if any, is not through modulation of sip or spb expression but rather by modification of the proteins.

Keywords: H-NS-like protein; Protein–protein interaction; Rhodobacter sphaeroides; SIP; SPB

Journal Article.  3014 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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