Journal Article

Contribution of Anaphase B to Chromosome Separation in Higher Plant Cells Estimated by Image Processing

Tomomi Hayashi, Toshio Sano, Natsumaro Kutsuna, Fumi Kumagai-Sano and Seiichiro Hasezawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 10, pages 1509-1513
Published in print October 2007 | ISSN: 0032-0781
Published online October 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm117
Contribution of Anaphase B to Chromosome Separation in Higher Plant Cells Estimated by Image Processing

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Anaphase can be categorized into the two subphases of anaphase A and B, but anaphase B has not been clearly described in higher plant cells. In this study, we time-sequentially followed the dynamics of chromosome segregation and spindle elongation in tobacco BY-2 cells using histone–red fluorescent protein (RFP) and green fluorescent protein (GFP)–tubulin, respectively. Construction of kymographs and determination of the positions of chromosomes and spindle edges by image processing revealed that anaphase B contributed to about 40% of the chromosome separation in distance, which is comparable with that in animal cells. These results suggest that higher plant cells potentially possess the process of anaphase B.

Keywords: Anaphase; Fluorescent proteins; Image processing

Journal Article.  2790 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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