Journal Article

Temperature Response of Photosynthesis in Transgenic Rice Transformed with ‘Sense’ or ‘Antisense’ <i>rbc</i>S

Amane Makino and Rowan F. Sage

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 10, pages 1472-1483
Published in print October 2007 | ISSN: 0032-0781
Published online October 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm118
Temperature Response of Photosynthesis in Transgenic Rice Transformed with ‘Sense’ or ‘Antisense’ rbcS

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The responses of chlorophyll fluorescence, gas exchange rate and Rubisco activation state to temperature were examined in transgenic rice plants with 130 and 35% of the wild-type (WT) Rubisco content by transformation with rbcS cDNA in sense and antisense orientations, respectively. Although the optimal temperatures of PSII quantum efficiency and CO2 assimilation were found to be between 25 and 32°C, the maximal activation state of Rubisco was found to be between 16 and 20°C in all genotypes. The Rubisco flux control coefficient was also the highest between 16 and 20°C in the WT and antisense lines [>0.88 at an intercellular CO2 pressure (Ci) of 28 Pa]. Gross photosynthesis at Ci = 28 Pa per Rubisco content in the WT between 12 and 20°C was close to that of the antisense lines where high Rubisco control is present. Thus, Rubisco activity most strongly limited photosynthesis at cool temperatures. These results indicated that a selective enhancement of Rubisco content can enhance photosynthesis at cool temperatures, but in the sense line with enhanced Rubisco content Pi regeneration limitation occurred. Above 20°C, the Rubisco flux control coefficient declined. This decline was associated with a decline in Rubisco activation. The activation state of Rubisco measured at each temperature decreased with increasing Rubisco content, and the slope of activation to Rubisco content was independent of temperature. We discuss the possibility that the decline in Rubisco activation at intermediate and high temperatures is part of a regulated response to a limitation in other photosynthetic processes.

Keywords: Gas exchange (leaf) — Oryza sativa L. — Rubisco — rbcS — Temperature response

Journal Article.  8297 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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