Journal Article

2-Hydroxyisoflavanone Dehydratase is a Critical Determinant of Isoflavone Productivity in Hairy Root Cultures of <i>Lotus japonicus</i>

Masayuki Shimamura, Tomoyoshi Akashi, Nozomu Sakurai, Hideyuki Suzuki, Kazuki Saito, Daisuke Shibata, Shin-ichi Ayabe and Toshio Aoki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 11, pages 1652-1657
Published in print November 2007 | ISSN: 0032-0781
Published online November 2007 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcm125
2-Hydroxyisoflavanone Dehydratase is a Critical Determinant of Isoflavone Productivity in Hairy Root Cultures of Lotus japonicus

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  • Biochemistry
  • Molecular and Cell Biology
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Hairy root cultures of a model legume, Lotus japonicus, were established to characterize two heterologous cDNAs encoding enzymes involved in isoflavone biosynthesis, i.e. licorice 2-hydroxyisoflavanone synthase (IFS) and soybean 2-hydroxyisoflavanone dehydratase (HID) catalyzing sequential reactions to yield isoflavones. While the control and the IFS overexpressor did not accumulate detectable isoflavones, the HID overexpressors did accumulate daidzein and genistein, showing that HID is a critical determinant of isoflavone productivity. Production of coumestrol in all the genotypes and isoliquiritigenin/liquiritigenin in IFS + HID-overexpressing lines was also noted. These results provide insight into the regulatory mechanism that controls isoflavonoid biosynthesis.

Keywords: Biosynthesis; Hairy root; 2-Hydroxyisoflavanone synthase; 2-Hydroxyisoflavanone dehydratase; Isoflavone; Lotus japonicus

Journal Article.  2928 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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