Journal Article

Purification and Characterization of UDP-Glucose : Curcumin Glucoside 1,6-Glucosyltransferase from <i>Catharanthus roseus</i> Cell Suspension Cultures

Yukie Oguchi, Sayaka Masada, Toshiya Kondo, Kazuyoshi Terasaka and Hajime Mizukami

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 11, pages 1635-1643
Published in print November 2007 | ISSN: 0032-0781
Published online November 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm138
Purification and Characterization of UDP-Glucose : Curcumin Glucoside 1,6-Glucosyltransferase from Catharanthus roseus Cell Suspension Cultures

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  • Biochemistry
  • Molecular and Cell Biology
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Catharanthus roseus cell suspension cultures converted exogenously added curcumin to a series of curcumin glucosides that possessed drastically enhanced water solubility. A cDNA clone encoding a glucosyltransferase responsible for glucosylation of curcumin to form curcumin 4′-O-glucoside was previously isolated, and in the present study a novel sugar–sugar glycosyltransferase, UDP-glucose:curcumin glucoside glucosyltransferase (UCGGT), was purified approximately 900-fold to apparent homogeneity from cultured cells of C. roseus. The purified enzyme (0.2% activity yield) catalyzed 1,6-glucosylation of curcumin 4′-O-glucoside to yield curcumin 4′-O-gentiobioside. The molecular weight and isoelectric point were estimated to be about 50 kDa and 5.2, respectively. The enzyme showed a pH optimum between 7.5 and 7.8. Both flavonoid 3-O- and 7-O-glucosides were also preferred acceptor substrates of the enzyme, whereas little activity was shown toward simple phenolic glucosides such as arbutin and glucovanillin, cyanogenic glucoside (prunasin) or flavonoid galactoside. These results suggest that UCGGT may also function in the biosynthesis of flavonoid glycosides in planta.

Keywords: Catharanthus roseus; Characterization; Curcumin glucoside; 1,6-Glucosyltransferase; Purification

Journal Article.  4626 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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