Journal Article

Plastidial Glutathione Reductase from <i>Haynaldia villosa</i> is an Enhancer of Powdery Mildew Resistance in Wheat (<i>Triticum aestivum</i>)

Ya-Ping Chen, Li-Ping Xing, Guo-Jiang Wu, Hua-Zhong Wang, Xiu-E Wang, Ai-Zhong Cao and Pei-Du Chen

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 48, issue 12, pages 1702-1712
Published in print December 2007 | ISSN: 0032-0781
Published online December 2007 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm142
Plastidial Glutathione Reductase from Haynaldia villosa is an Enhancer of Powdery Mildew Resistance in Wheat (Triticum aestivum)

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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A full-length cDNA (Hv-GR) whose transcript accumulation increased in response to infection by Blumeria graminis DC.f.sp. tritici (Bgt) was isolated from Haynaldia villosa. Southern analysis revealed a single copy of Hv-GR present in H. villosa. This gene encodes a glutathione reductase (GR) with high similarity to chloroplastic GRs from other plant species. Chloroplastic localization of Hv-GR was confirmed by targeting of the green fluorescent protein (GFP)–Hv-GR fusion protein to chloroplasts of epidermal guard cells. Following inoculation with Bgt, transcript accumulation of Hv-GR increased in a resistant line of wheat, but no significant change was observed in a susceptible line. In vivo function of Hv-GR in converting oxidized glutathione (GSSG) to the reduced form (GSH) was verified through heterologous expression of Hv-GR in a yeast GR-deficient mutant. As expected, overexpression of this gene resulted in increased resistance of the mutant to H2O2, indicating a critical role for Hv-GR in protecting cells against oxidative stress. Moreover, overexpression of Hv-GR in a susceptible wheat variety, Triticum aestivum cv.Yangmai 158, enhanced resistance to powdery mildew and induced transcript accumulation of other pathogenesis-related genes, PR-1a and PR-5, through increasing the foliar GSH/GSSG ratio. Therefore, we concluded that a high ratio of GSH to GSSG is required for wheat defense against Bgt, and that chloroplastic GR enzymes might serve as a redox mediator for NPR1 activation.

Keywords: Chloroplastic glutathione reductase; Disease resistance; Haynaldia villosa; Powdery mildew; Triticum aestivum

Journal Article.  5336 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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