Journal Article

Polyethylene Glycol (PEG)-Mediated Transient Gene Expression in a Red Alga, <i>Cyanidioschyzon merolae</i> 10D

Mio Ohnuma, Takashi Yokoyama, Takayuki Inouye, Yasuhiko Sekine and Kan Tanaka

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 1, pages 117-120
Published in print January 2008 | ISSN: 0032-0781
Published online January 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcm157
Polyethylene Glycol (PEG)-Mediated Transient Gene Expression in a Red Alga, Cyanidioschyzon merolae 10D

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  • Biochemistry
  • Molecular and Cell Biology
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DNA introduction into cells is an essential technique for molecular genetic analysis. Here, we show that DNA is easily introduced into cells of the unicellular red alga Cyanidioschyzon merolae by a polyethylene glycol (PEG)-mediated protocol. In this study, the β-tubulin gene of C. merolae was cloned on a plasmid and a hemagglutinin (HA) tag then added at the C-terminus. This plasmid was then introduced into C. merolae cells by a PEG-mediated transformation protocol. At 24 h after PEG-mediated transformation, intracellular localization of the tagged protein was detected by anti-HA immunocytochemistry, indicating the utility of this transient expression system for molecular genetic analyses.

Keywords: Cyanidioschyzon merolae; Hemagglutinin; Polyethylene glycol; Transformation; Transient expression system

Journal Article.  2507 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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