Journal Article

Defense-Related Signaling by Interaction of Arabinogalactan Proteins and β-Glucosyl Yariv Reagent Inhibits Gibberellin Signaling in Barley Aleurone Cells

Kiyoshi Mashiguchi, Eriko Urakami, Morifumi Hasegawa, Kazutsuka Sanmiya, Ichiro Matsumoto, Isomaro Yamaguchi, Tadao Asami and Yoshihito Suzuki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 2, pages 178-190
Published in print February 2008 | ISSN: 0032-0781
Published online February 2008 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcm175
Defense-Related Signaling by Interaction of Arabinogalactan Proteins and β-Glucosyl Yariv Reagent Inhibits Gibberellin Signaling in Barley Aleurone Cells

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Arabinogalactan proteins (AGPs) are hydroxyproline-rich glycoproteins present at the plasma membrane and in extracellular spaces. A synthetic chemical, β-glucosyl Yariv reagent (β-GlcY), binds specifically to AGPs. We previously reported that gibberellin signaling is specifically inhibited by β-GlcY treatment in barley aleurone protoplasts. In the present study, we found that β-GlcY also inhibited gibberellin-induced programmed cell death (PCD) in aleurone cells. We examined the universality and specificity of the inhibitory effect of β-GlcY on gibberellin signaling using microarray analysis and found that β-GlcY was largely effective in repressing gibberellin-induced gene expression. In addition, >100 genes were up-regulated by β-GlcY in a gibberellin-independent manner, and many of these were categorized as defense-related genes. Defense signaling triggered by several defense system inducers such as jasmonic acid and a chitin elicitor could inhibit gibberellin-inducible events such as α-amylase secretion, PCD and expression of some gibberellin-inducible genes in aleurone cells. Furthermore, β-GlcY repressed the gibberellin-inducible Ca2+-ATPase gene which is important for gibberellin-dependent gene expression, and induced known repressors of gibberellin signaling, two WRKY genes and a NAK kinase gene. These effects of β-GlcY were also phenocopied by the chitin elicitor and/or jasmonic acid. These results indicate that gibberellin signaling is under the regulation of defense-related signaling in aleurone cells. It is also probable that AGPs are involved in the perception of stimuli causing defense responses.

Keywords: Aleurone layer — Arabinogalactan protein — Barley — Defense response — Gibberellin — Protoplast

Journal Article.  7324 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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