Journal Article

A Novel <i>orf108</i> Co-Transcribed with the <i>atpA</i> Gene is Associated with Cytoplasmic Male Sterility in <i>Brassica juncea</i> Carrying <i>Moricandia arvensis</i> Cytoplasm

Ashutosh, Pankaj Kumar, V. Dinesh Kumar, Prakash C. Sharma, Shyam Prakash and Shripad R. Bhat

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 2, pages 284-289
Published in print February 2008 | ISSN: 0032-0781
Published online February 2008 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcm182
A Novel orf108 Co-Transcribed with the atpA Gene is Associated with Cytoplasmic Male Sterility in Brassica juncea Carrying Moricandia arvensis Cytoplasm

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  • Biochemistry
  • Molecular and Cell Biology
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Mitochondrial atpA transcripts were examined in cytoplasmic male sterile (CMS) and fertility restorer lines of CMS (Moricandia arvensis) Brassica juncea. Male sterile flowers had longer atpA transcripts than male fertiles. The mitochondrial atpA region of the CMS line was cloned and sequenced. The 5′ and 3′ ends of the atpA transcripts of the CMS and the fertility restorer lines were mapped and full-length transcripts were cloned and sequenced. A novel orf108 (open reading frame 108) co-transcribed with the atpA gene was found in the male sterile flowers. In the fertility restorer line, the transcript was cleaved within orf108 to yield monocistronic atpA transcripts.

Keywords: atpA; Brassica juncea; Cytoplasmic male sterility; Fertility restoration; orf108; Transcript mapping

Journal Article.  3085 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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