Journal Article

Ribosome Stacking Defines <i>CGS1</i> mRNA Degradation Sites During Nascent Peptide-Mediated Translation Arrest

Yuhi Haraguchi, Yoshitomo Kadokura, Mari Nakamoto, Hitoshi Onouchi and Satoshi Naito

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 3, pages 314-323
Published in print March 2008 | ISSN: 0032-0781
Published online March 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn005
Ribosome Stacking Defines CGS1 mRNA Degradation Sites During Nascent Peptide-Mediated Translation Arrest

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Expression of the Arabidopsis CGS1 gene that codes for cystathionine γ-synthase is feedback-regulated at the step of mRNA degradation in response to S-adenosyl-l-methionine (AdoMet). This regulation occurs during translation and involves AdoMet-induced temporal translation arrest prior to the mRNA degradation. Here, we have identified multiple intermediates of CGS1 mRNA degradation with different 5′ ends that are separated by approximately 30 nucleotides. Longer intermediates were found to be produced as the number of ribosomes loaded on mRNA was increased. Sucrose density gradient centrifugation experiments showed that the shortest mRNA degradation intermediate was associated with monosomes, whereas longer degradation intermediates were associated with multiple ribosomes. Immunoblot analyses revealed a ladder of premature polypeptides whose molecular weights corresponded to products of ribosomes in a stalled stack. An increase in smaller premature polypeptides was observed as the number of ribosomes loaded on mRNA increased. These results show that AdoMet induces the stacking of ribosomes on CGS1 mRNA and that multiple mRNA degradation sites probably correspond to each stacked ribosome.

Keywords: S-Adenosyl-l-methionine; Arabidopsis thaliana; Feedback regulation; Methionine biosynthesis; mRNA stability; MTO1 region

Journal Article.  5531 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.