Journal Article

Functional Differentiation of <i>Lotus japonicus</i> TT2s, R2R3-MYB Transcription Factors Comprising a Multigene Family

Kazuko Yoshida, Rieko Iwasaka, Takakazu Kaneko, Shusei Sato, Satoshi Tabata and Masaaki Sakuta

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 2, pages 157-169
Published in print February 2008 | ISSN: 0032-0781
Published online February 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn009
Functional Differentiation of Lotus japonicus TT2s, R2R3-MYB Transcription Factors Comprising a Multigene Family

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Leguminous plants have many paralogous genes encoding enzymes involved in the flavonoid biosynthetic pathway. Duplicate genes are predicted to contribute to the production of various flavonoid compounds and to have resulted in a diversity of legume species. We identified gene duplication in the transcription factors regulating flavonoid biosynthesis in the model legume Lotus japonicus. Three copies of a homolog of Arabidopsis thaliana TRANSPARENT TESTA2 (TT2), which is a MYB transcription factor that regulates proanthocyanidin biosynthesis, were present in the L. japonicus genome. The organ specificity and stress responsiveness differed among the three LjTT2s, and correlations between proanthocyanidin accumulation and the expression levels of LjTT2s were observed during seedling development. Moreover, three LjTT2s functionally complemented TT2 in transient expression experiments in A. thaliana leaf cells. The different reporter activity caused by LjTT2a was consistent with the affinity of physical interactions with TT8 and TTG1 in yeast two-hybrid experiments as well as the branching pattern of the phylogenetic tree. These results suggest that LjTT2 factors have diverse functions in the tissues in which they are expressed; in particular, LjTT2a is predicted to have evolved flexibility in interaction with other transcription regulators to resist environmental stresses.

Keywords: Lotus japonicus; Multigene family; MYB; Proanthocyanidin; Transcription factor

Journal Article.  6868 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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