Journal Article

The Necrotroph <i>Botrytis cinerea</i> Induces a Non-Host Type II Resistance Mechanism in <i>Pinus pinaster</i> Suspension-Cultured Cells

Herlânder Azevedo, Teresa Lino-Neto and Rui Manuel Tavares

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 3, pages 386-395
Published in print March 2008 | ISSN: 0032-0781
Published online March 2008 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcn015
The Necrotroph Botrytis cinerea Induces a Non-Host Type II Resistance Mechanism in Pinus pinaster Suspension-Cultured Cells

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Models of non-host resistance have failed to account for the pathogenicity of necrotrophic agents. During the interaction of Pinus pinaster (maritime pine) with the non-host necrotrophic pathogen Botrytis cinerea, the generation and scavenging of reactive oxygen species (ROS) and the induction of the hypersensitive response (HR) were analyzed. Elicitation of maritime pine suspended cells with B. cinerea spores resulted in the biphasic induction of ROS. The phase I oxidative burst was dependent on calcium influx, while the phase II oxidative burst also depended on NADPH oxidase, protein kinase activity, and de novo transcription and protein synthesis. A decline was observed in catalase (CAT) and superoxide dismutase (SOD) activity, together with the down-regulation of Fe-Sod1, chlCu, Zn-Sod1 and csApx1, suggesting a coordinated response towards a decrease in the ROS-scavenging capacity of maritime pine cells during challenge. Following the second oxidative burst, programmed cell death events characteristic of the HR were observed. The results suggest the ROS-mediated and cell-breach-independent activation of Type II non-host resistance during the P. pinasterB. cinerea interaction.

Keywords: Botrytis cinerea; Necrotrophic fungi; Non-host Type II resistance; Pinus pinaster; ROS

Journal Article.  6754 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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