Journal Article

Identification of Genes Expressed in Vascular Tissues Using NPA-Induced Vascular Overgrowth in <i>Arabidopsis</i>

Carol L. Wenzel, Qian Hester and Jim Mattsson

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 3, pages 457-468
Published in print March 2008 | ISSN: 0032-0781
Published online March 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn023
Identification of Genes Expressed in Vascular Tissues Using NPA-Induced Vascular Overgrowth in Arabidopsis

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The genetic basis of vascular differentiation and function is relatively poorly understood, partly due to the difficulty of screening for mutants defective in internal vascular tissues. Here we present an approach based on a predicted increase in vascular-related gene expression in response to an auxin transport inhibitor-induced vascular overgrowth. We used microarray analyses to identify 336 genes that were up-regulated 2-fold in shoot tissues of Arabidopsis thaliana showing vascular overgrowth. Promoter–marker gene fusions revealed that 38 out of 40 genes with 4-fold up-regulation in vascular overgrowth tissues had vascular-related expression in transgenic Arabidopsis plants. Obtained expression patterns included cambial tissues and differentiating xylem, phloem and fibers. A total of 15 genes were found to have vascular-specific expression patterns in the leaves and/or inflorescence stems. This study provides empirical evidence of the efficiency of the approach and describes for the first time the in situ expression patterns of the majority of the assessed genes.

Keywords: Auxin; Vascular expression; Vascular overgrowth

Journal Article.  5437 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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