Journal Article

Identification and Localization of the CupB Protein Involved in Constitutive CO<sub>2</sub> Uptake in the Cyanobacterium, <i>Synechocystis</i> sp. Strain PCC 6803

Min Xu, Teruo Ogawa, Himadri B. Pakrasi and Hualing Mi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 6, pages 994-997
Published in print June 2008 | ISSN: 0032-0781
Published online June 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn074
Identification and Localization of the CupB Protein Involved in Constitutive CO2 Uptake in the Cyanobacterium, Synechocystis sp. Strain PCC 6803

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Antibody against cMyc cross-reacted strongly with the CupB protein tagged with His6-cMyc (HM) in thylakoid membrane of Synechocystis sp. strain PCC 6803 but only faintly with the cytoplasmic membrane fraction. The protein was not detected in the membranes of the ΔndhD4 and ΔndhF4 mutants in which CupB was tagged with HM. We concluded that a CupB complex containing NdhD4 and NdhF4 is largely, if not exclusively, confined to the thylakoid membrane. Both CupB and NdhH were detected in a fraction containing protein complexes of > 450 kDa, obtained after nickel column and gel filtration chromatography of the membranes solubilized with n-dodecyl-β-maltoside.

Keywords: CO2 uptake; CupB; Cytoplasmic membrane; NDH-1; Synechocystis 6803; Thylakoid membrane

Journal Article.  2301 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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