Journal Article

Mitochondrial Dynamics in Plant Male Gametophyte Visualized by Fluorescent Live Imaging

Ryo Matsushima, Yuki Hamamura, Tetsuya Higashiyama, Shin-ichi Arimura, Sodmergen, Nobuhiro Tsutsumi and Wataru Sakamoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 7, pages 1074-1083
Published in print July 2008 | ISSN: 0032-0781
Published online June 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn084
Mitochondrial Dynamics in Plant Male Gametophyte Visualized by Fluorescent Live Imaging

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Visualization of organelles in living cells is a powerful method for studying their dynamic behavior. Here we attempted to visualize mitochondria in angiosperm male gametophyte (pollen grain from Arabidopsis thaliana) that are composed of one vegetative cell (VC) and two sperm cells (SCs). Combination of mitochondria-targeted fluorescent proteins with VC- or SC-specific expression allowed us to observe the precise number and dynamic behavior of mitochondria in the respective cell types. Furthermore, live imaging of SC mitochondria during double fertilization confirmed previous observations, demonstrated by electron microscopy in other species, that sperm mitochondria enter into the egg and central cells. We also attempted to visualize mutant mitochondria that were elongated due to a defect in mitochondrial division. This mutant phenotype was indeed detectable in VC mitochondria of a heterozygous F1 plant, suggesting active mitochondrial division in male gametophyte. Finally, we performed mutant screening and isolated a putative mitochondrial protein transport mutant whose phenotype was detectable only in haploid cells. The transgenic materials presented in this work are useful not only for live imaging but also for studying mitochondrial functions by mutant analysis.

Keywords: Arabidopsis thaliana; Fertilization; Fluorescent protein; Mitochondria; Pollen grain; Sperm cell

Journal Article.  5152 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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