Journal Article

Effects of Polylinker uATGs on the Function of Grass HKT1 Transporters Expressed in Yeast Cells

María A. Bañuelos, Rosario Haro, Ana Fraile-Escanciano and Alonso Rodríguez-Navarro

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 7, pages 1128-1132
Published in print July 2008 | ISSN: 0032-0781
Published online June 2008 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcn088
Effects of Polylinker uATGs on the Function of Grass HKT1 Transporters Expressed in Yeast Cells

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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HvHKT1 mediates K+ or Na+ uniport in yeast cells if the expression promoter is joined directly to the HvHKT1 cDNA, and Na+–K+ symport if a 59 nucleotide polylinker is inserted. Our results show that three ATG triplets in the polylinker decreased the synthesis of the transporter and that the lower amount of transporter caused the functional change. With the rice HKT1 cDNA, the 59 nt polylinker changed the mode of Na+ uptake from K+-insensitive to K+-inhibitable. These two modes of Na+ uptake also occurred in rice plants.

Keywords: Plant; Potassium; Sodium; Transport; uORFs; Yeast

Journal Article.  2863 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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