Journal Article

Live Cell Imaging Reveals Plant Aurora Kinase Has Dual Roles During Mitosis

Daisuke Kurihara, Sachihiro Matsunaga, Susumu Uchiyama and Kiichi Fukui

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 8, pages 1256-1261
Published in print August 2008 | ISSN: 0032-0781
Published online July 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn098
Live Cell Imaging Reveals Plant Aurora Kinase Has Dual Roles During Mitosis

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The proper segregation of chromosomes during mitosis is required for accurate distribution of genetic information by two daughter cells. Here, we used live cell imaging of microtubules and kinetochores after treatment with an Aurora kinase inhibitor, hesperadin, in tobacco BY-2 cells to analyze the function of plant Aurora kinase during mitosis. Hesperadin treatment induced the delay of CenH3 alignment on the spindle equator. Furthermore, two types of dynamics of lagging CenH3s were observed during chromosome segregation. The findings indicate that the plant Aurora kinase has dual roles; correction of aberrant kinetochore–microtubule attachment and dissociation of cohesin during chromosome alignment and segregation.

Keywords: Chromosome segregation; Cohesin dissociation; Kinetochore–microtubule attachment; Lagging chromosome; Plant Aurora kinase

Journal Article.  2869 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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