Journal Article

The Glycerophosphoryl Diester Phosphodiesterase-Like Proteins SHV3 and its Homologs Play Important Roles in Cell Wall Organization

Shimpei Hayashi, Tadashi Ishii, Toshiro Matsunaga, Rumi Tominaga, Takashi Kuromori, Takuji Wada, Kazuo Shinozaki and Takashi Hirayama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 10, pages 1522-1535
Published in print October 2008 | ISSN: 0032-0781
Published online August 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn120
The Glycerophosphoryl Diester Phosphodiesterase-Like Proteins SHV3 and its Homologs Play Important Roles in Cell Wall Organization

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Despite the importance of extracellular events in cell wall organization and biogenesis, the mechanisms and related factors are largely unknown. We isolated an allele of the shaven3 (shv3) mutant of Arabidopsis thaliana, which exhibits ruptured root hair cells during tip growth. SHV3 encodes a novel protein with two tandemly repeated glycerophosphoryl diester phosphodiesterase-like domains and a glycosylphosphatidylinositol anchor, and several of its paralogs are found in Arabidopsis. Here, we report the detailed characterization of mutants of SHV3 and one of its paralogs, SVL1. The shv3 and svl1 double mutant exhibited additional defects, including swollen guard cells, aberrant expansion of the hypocotyl epidermis and ectopic lignin deposits, suggesting decreased rigidity of the cell wall. Fourier-transform infrared spectroscopy and measurement of the cell wall components indicated an altered cellulose content and pectin modification with cross-linking in the double mutant. Furthermore, we found that the ruptured root hair phenotype of shv3 was suppressed by increasing the amount of borate, which is supposed to be involved in pectic polysaccharide cross-linking, in the medium. These findings indicate that SHV3 and its paralogs are novel important factors involved in primary cell wall organization.

Keywords: Arabidopsis thaliana; Cell wall; Cellulose; GPI-anchored protein; Pectin; Root hair

Journal Article.  7944 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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