Journal Article

Separated Transcriptomes of Male Gametophyte and Tapetum in Rice: Validity of a Laser Microdissection (LM) Microarray

Keita Suwabe, Go Suzuki, Hirokazu Takahashi, Katsuhiro Shiono, Makoto Endo, Kentaro Yano, Masahiro Fujita, Hiromi Masuko, Hiroshi Saito, Tomoaki Fujioka, Fumi Kaneko, Tomohiko Kazama, Yoko Mizuta, Makiko Kawagishi-Kobayashi, Nobuhiro Tsutsumi, Nori Kurata, Mikio Nakazono and Masao Watanabe

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 10, pages 1407-1416
Published in print October 2008 | ISSN: 0032-0781
Published online August 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn124
Separated Transcriptomes of Male Gametophyte and Tapetum in Rice: Validity of a Laser Microdissection (LM) Microarray

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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In flowering plants, the male gametophyte, the pollen, develops in the anther. Complex patterns of gene expression in both the gametophytic and sporophytic tissues of the anther regulate this process. The gene expression profiles of the microspore/pollen and the sporophytic tapetum are of particular interest. In this study, a microarray technique combined with laser microdissection (44K LM-microarray) was developed and used to characterize separately the transcriptomes of the microspore/pollen and tapetum in rice. Expression profiles of 11 known tapetum specific-genes were consistent with previous reports. Based on their spatial and temporal expression patterns, 140 genes which had been previously defined as anther specific were further classified as male gametophyte specific (71 genes, 51%), tapetum-specific (seven genes, 5%) or expressed in both male gametophyte and tapetum (62 genes, 44%). These results indicate that the 44K LM-microarray is a reliable tool to analyze the gene expression profiles of two important cell types in the anther, the microspore/pollen and tapetum.

Keywords: Anther; Laser microdissection; Microarray; Oryza sativa; L. Microspore/pollen; Tapetum

Journal Article.  4921 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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