Journal Article

Anion Channel Activation and Proton Pumping Inhibition Involved in the Plasma Membrane Depolarization Induced by ABA in <i>Arabidopsis thaliana</i> Suspension Cells are Both ROS Dependent

Jacques Trouverie, Guillaume Vidal, Zongshen Zhang, Caroline Sirichandra, Karine Madiona, Zahia Amiar, Jean-Louis Prioul, Emmanuelle Jeannette, Jean-Pierre Rona and Mathias Brault

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 10, pages 1495-1507
Published in print October 2008 | ISSN: 0032-0781
Published online August 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn126
Anion Channel Activation and Proton Pumping Inhibition Involved in the Plasma Membrane Depolarization Induced by ABA in Arabidopsis thaliana Suspension Cells are Both ROS Dependent

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  • Biochemistry
  • Molecular and Cell Biology
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In Arabidopsis thaliana suspension cells, ABA was previously shown to promote the activation of anion channels and the reduction of proton pumping that both contribute to the plasma membrane depolarization. These two ABA responses were shown to induce two successive [Ca2+]cyt spikes. As reactive oxygen species (ROS) have emerged as components of ABA signaling pathways especially by promoting [Ca2+]cyt variations, we studied whether ROS were involved in the regulation of anion channels and proton pumps activities. Here we demonstrated that ABA induced ROS production which triggered the second of the two [Ca2+]cyt increases observed in response to ABA. Blocking ROS generation using diphenyleneiodonium (DPI) impaired the proton pumping reduction, the anion channel activation and the RD29A gene expression in response to ABA. Furthermore, H2O2 was shown to activate anion channels and to inhibit plasma membrane proton pumping, as did ABA. However, ROS partially mimicked ABA's effects since H2O2 treatment elicited anion channel activation but not the subsequent expression of the RD29A gene as did ABA. This suggests that expression of the RD29A gene in response to ABA results from the activation of multiple concomitant signaling pathways: blocking of one of them would impair gene expression whereas stimulating only one would not. We conclude that ROS are a central messenger of ABA in the signaling pathways leading to the plasma membrane depolarization induced by ABA.

Keywords: ABA; Anion channel; Arabidopsis thaliana; Calcium; Proton pump; ROS

Journal Article.  7717 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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