Journal Article

Co-Expression of Soybean Glycinins A1aB1b and A3B4 Enhances Their Accumulation Levels in Transgenic Rice Seed

Fumio Takaiwa, Chiyoko Sakuta, Seon-Kang Choi, Yoshifumi Tada, Takayasu Motoyama and Shigeru Utsumi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 10, pages 1589-1599
Published in print October 2008 | ISSN: 0032-0781
Published online September 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn135
Co-Expression of Soybean Glycinins A1aB1b and A3B4 Enhances Their Accumulation Levels in Transgenic Rice Seed

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The soybean major storage protein glycinin is encoded by five genes, which are divided into two subfamilies. Expression of A3B4 glycinin in transgenic rice seed reached about 1.5% of total seed protein, even if expressed under the control of strong endosperm-specific promoters. In contrast, expression of A1aB1b glycinin reached about 4% of total seed protein. Co-expression of the two proteins doubled accumulation levels of both A1aB1b and A3B4 glycinins. This increase can be largely accounted for by their aggregation with rice glutelins, self-assembly and inter-glycinin interactions, resulting in the enrichment of globulin and glutelin fractions and a concomitant reduction of the prolamin fraction. Immunoelectron microscopy indicated that the synthesized A1aB1b glycinin was predominantly deposited in protein body-II (PB-II) storage vacuoles, whereas A3B4 glycinin is targeted to both PB-II and endoplasmic reticulum (ER)-derived protein body-I (PB-I) storage structures. Co-expression with A1aB1b facilitated targeting of A3B4 glycinin into PB-II by sequestration with A1aB1b, resulting in an increase in the accumulation of A3B4 glycinin.

Keywords: Endosperm; Glycinin; Immunolocalization; Protein body; Seed storage protein; Transgenic rice

Journal Article.  6620 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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