Journal Article

Properties of Mutants of <i>Synechocystis</i> sp. Strain PCC 6803 Lacking Inorganic Carbon Sequestration Systems

Min Xu, Gábor Bernát, Abhay Singh, Hualing Mi, Matthias Rögner, Himadri B. Pakrasi and Teruo Ogawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 11, pages 1672-1677
Published in print November 2008 | ISSN: 0032-0781
Published online September 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn139
Properties of Mutants of Synechocystis sp. Strain PCC 6803 Lacking Inorganic Carbon Sequestration Systems

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

A mutant (Δ5) of Synechocystis sp. strain PCC 6803 constructed by inactivating five inorganic carbon sequestration systems did not take up CO2 or HCO3 and was unable to grow in air with or without glucose. The Δ4 mutant in which BicA is the only active inorganic carbon sequestration system showed low activity of HCO3 uptake and grew under these conditions but more slowly than the wild-type strain. The Δ5 mutant required 1.7% CO2 to attain half the maximal growth rate. Electron transport activity of the mutants was strongly inhibited under high light intensities, with the Δ5 mutant more susceptible to high light than the Δ4 mutant. The results implicated the significance of carbon sequestration in dissipating excess light energy.

Keywords: CO2 and HCO3− uptake; CO2-concentrating mechanism (CCM); Cyanobacteria; Electron transport; Inorganic carbon sequestration

Journal Article.  3978 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.