Journal Article

Degradation of Sphingoid Long-Chain Base 1-Phosphates (LCB-1Ps): Functional Characterization and Expression of <i>AtDPL1</i> Encoding LCB-1P Lyase Involved in the Dehydration Stress Response in <i>Arabidopsis</i>

Masahiro Nishikawa, Kenta Hosokawa, Mai Ishiguro, Hiroki Minamioka, Kentaro Tamura, Ikuko Hara-Nishimura, Yohei Takahashi, Ken-ichiro Shimazaki and Hiroyuki Imai

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 11, pages 1758-1763
Published in print November 2008 | ISSN: 0032-0781
Published online October 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn149
Degradation of Sphingoid Long-Chain Base 1-Phosphates (LCB-1Ps): Functional Characterization and Expression of AtDPL1 Encoding LCB-1P Lyase Involved in the Dehydration Stress Response in Arabidopsis

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Sphingoid long-chain base (LCB) 1-phosphates are degradated by LCB 1-phosphate lyase to C16 fatty aldehydes and phosphoethanolamine. Here, we confirmed that the At1g27980 gene product, AtDPL1, is a functional LCB-1-phosphate lyase. Expression of green fluorescent protein fusion products in suspension-cultured Arabidopsis cells showed that AtDPL1 is located to the endoplasmic reticulum. The rates of fresh weight decreases of dpl1-1 and dpl1-2 mutants were significantly slower than those of the wild-type plants. This ability to limit their transpiration reflected the leaf temperature of the mutant plants more than that of wild-type plants, suggesting that AtDPL1 plays a role in dehydration stress.

Keywords: Dehydration stress; Long-chain base; Sphingosine-1-phosphate lyase; Transpiration

Journal Article.  3231 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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