Journal Article

PKS Activities and Biosynthesis of Cannabinoids and Flavonoids in <i>Cannabis sativa</i> L. Plants

Isvett Josefina Flores-Sanchez and Robert Verpoorte

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 12, pages 1767-1782
Published in print December 2008 | ISSN: 0032-0781
Published online December 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn150
PKS Activities and Biosynthesis of Cannabinoids and Flavonoids in Cannabis sativa L. Plants

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Polyketide synthase (PKS) enzymatic activities were analyzed in crude protein extracts from cannabis plant tissues. Chalcone synthase (CHS, EC 2.3.1.74), stilbene synthase (STS, EC 2.3.1.95), phlorisovalerophenone synthase (VPS, EC 2.3.1.156), isobutyrophenone synthase (BUS) and olivetol synthase activities were detected during the development and growth of glandular trichomes on bracts. Cannabinoid biosynthesis and accumulation take place in these glandular trichomes. In the biosynthesis of the first precursor of cannabinoids, olivetolic acid, a PKS could be involved; however, no activity for an olivetolic acid-forming PKS was detected. Content analyses of cannabinoids and flavonoids, two secondary metabolites present in this plant, from plant tissues revealed differences in their distribution, suggesting a diverse regulatory control for these biosynthetic fluxes in the plant.

Keywords: Cannabinoids; Cannabis sativa; Flavonoids; Glandular trichomes; Olivetol synthase; Polyketide synthase

Journal Article.  7656 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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