Journal Article

Interplay of RNA Pol IV and ROS1 During Post-Embryonic 5S rDNA Chromatin Remodeling

Julien Douet, Bertrand Blanchard, Claudine Cuvillier and Sylvette Tourmente

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 49, issue 12, pages 1783-1791
Published in print December 2008 | ISSN: 0032-0781
Published online December 2008 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcn152
Interplay of RNA Pol IV and ROS1 During Post-Embryonic 5S rDNA Chromatin Remodeling

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We have investigated the chromatin structure of 5S rDNA, a heterochromatic pericentromeric tandemly repeated family, at 2, 3, 4 and 5 days post-germination. Our results revealed a large-scale reorganization of 5S rDNA chromatin that occurs during the first days of development. Unexpectedly, there is a decondensation followed by a ‘re’condensation of 5S rDNA chromatin, to obtain almost mature nuclei 5 d post-germination. The reorganization of 5S rDNA chromatin is accompanied by a rapid and active demethylation of 5S rDNA mediated by the ROS1 (repressor of silencing 1) demethylase, whereas the plant-specific RNA polymerase IV (Pol IV) is essential to the 5S chromatin ‘re’condensation. In conclusion, Pol IV and ROS1 collaborate to unlock the 5S rDNA chromatin inherited from the seed, and establish adult features.

Keywords: Arabidopsis thaliana; Chromatin; RNA polymerase IV; ROS1; 5S rDNA

Journal Article.  4927 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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